Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain
Joint Authors
Martín-Martín, Inés
Molina, Ricardo
Jiménez, Maribel
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-22
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit.
Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection.
In order to overcome these difficulties, producing recombinant proteins of different vectors has become a major task.
In this study, a cDNA library was constructed with the salivary glands of Phlebotomus perniciosus from Madrid, Spain, the most widespread vector of Leishmania infantum in the Mediterranean basin.
Analysis of the cDNA sequences showed several polymorphisms among the previously described salivary transcripts.
The apyrase SP01B and the D7-related protein SP04 were successfully cloned, expressed in Escherichia coli, and purified.
Besides, recombinant proteins were recognized by sera of hamsters and mice previously immunized with saliva through the exposure to uninfected sand fly bites.
These results suggest that these two recombinant proteins conserved their immunogenic properties after expression in a prokaryote system.
Therefore, this work contributes to expand the knowledge of P.
perniciosus saliva that would be eventually used for the development of tools for vector control programs.
American Psychological Association (APA)
Martín-Martín, Inés& Molina, Ricardo& Jiménez, Maribel. 2013. Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain. BioMed Research International،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1004512
Modern Language Association (MLA)
Martín-Martín, Inés…[et al.]. Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain. BioMed Research International No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-1004512
American Medical Association (AMA)
Martín-Martín, Inés& Molina, Ricardo& Jiménez, Maribel. Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1004512
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004512