![](/images/graphics-bg.png)
Poly(lactic-co-glycolic) AcidNanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells
Joint Authors
Wang, Lin
Li, Chunyan
Chen, Yingxin
Dong, Shujun
Chen, Xuesi
Zhou, Yanmin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Adrenomedullin (ADM) is a bioactive regulatory peptide that affects migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells.
This study investigated the effects of sustained release of ADM on the modulation activity of osteoblasts and vascular endothelial cells in vitro.
Chitosan microspheres (CMs) were developed for ADM delivery.
Poly(lactic-co-glycolic) acid and nano-hydroxyapatite were used to prepare scaffolds containing microspheres with ADM.
The CMs showed rough surface morphology and high porosity, and they were well-distributed.
The scaffolds exhibited relatively uniform pore sizes with interconnected pores.
The addition of CMs improved the mechanical properties of the scaffolds without affecting their high porosity.
In vitro degradation tests indicated that the addition of CMs increased the water absorption of the scaffolds and inhibited pH decline of phosphate-buffered saline medium.
The expression levels of osteogenic-related and angiogenic-related genes were determined in MG63 cells and in human umbilical vein endothelial cells cultured on the scaffolds, respectively.
The expression levels of osteogenic-related and angiogenic-related proteins were also detected by western blot analysis.
Their expression levels in cells were improved on the ADM delivery scaffolds at a certain time point.
The in vitro evaluation suggests that the microsphere-scaffold system is suitable as a model for bone tissue engineering.
American Psychological Association (APA)
Wang, Lin& Li, Chunyan& Chen, Yingxin& Dong, Shujun& Chen, Xuesi& Zhou, Yanmin. 2013. Poly(lactic-co-glycolic) AcidNanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1004538
Modern Language Association (MLA)
Wang, Lin…[et al.]. Poly(lactic-co-glycolic) AcidNanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1004538
American Medical Association (AMA)
Wang, Lin& Li, Chunyan& Chen, Yingxin& Dong, Shujun& Chen, Xuesi& Zhou, Yanmin. Poly(lactic-co-glycolic) AcidNanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1004538
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004538