Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae)
Joint Authors
Park, Seong Hwan
Park, Chung Hyun
Zhang, Yong
Piao, Huguo
Chung, Ukhee
Kim, Seong Yoon
Ko, Kwang Soo
Yi, Cheong-Ho
Jo, Tae-Ho
Hwang, Juck-Joon
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Identifying species of insects used to estimate postmortem interval (PMI) is a major subject in forensic entomology.
Because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, DNA markers are greatly needed.
To develop new autosomal DNA markers to identify species, partial genomic sequences of the bicoid (bcd) genes, containing the homeobox and its flanking sequences, from 12 blowfly species (Aldrichina grahami, Calliphora vicina, Calliphora lata, Triceratopyga calliphoroides, Chrysomya megacephala, Chrysomya pinguis, Phormia regina, Lucilia ampullacea, Lucilia caesar, Lucilia illustris, Hemipyrellia ligurriens and Lucilia sericata; Calliphoridae: Diptera) were determined and analyzed.
This study first sequenced the ten blowfly species other than C.
vicina and L.
sericata.
Based on the bcd sequences of these 12 blowfly species, a phylogenetic tree was constructed that discriminates the subfamilies of Calliphoridae (Luciliinae, Chrysomyinae, and Calliphorinae) and most blowfly species.
Even partial genomic sequences of about 500 bp can distinguish most blowfly species.
The short intron 2 and coding sequences downstream of the bcd homeobox in exon 3 could be utilized to develop DNA markers for forensic applications.
These gene sequences are important in the evolution of insect developmental biology and are potentially useful for identifying insect species in forensic science.
American Psychological Association (APA)
Park, Seong Hwan& Park, Chung Hyun& Zhang, Yong& Piao, Huguo& Chung, Ukhee& Kim, Seong Yoon…[et al.]. 2013. Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae). BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004550
Modern Language Association (MLA)
Park, Seong Hwan…[et al.]. Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae). BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004550
American Medical Association (AMA)
Park, Seong Hwan& Park, Chung Hyun& Zhang, Yong& Piao, Huguo& Chung, Ukhee& Kim, Seong Yoon…[et al.]. Using the Developmental Gene Bicoid to Identify Species of Forensically Important Blowflies (Diptera: Calliphoridae). BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004550
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004550