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The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role in Trichomonas vaginalis Cytoadherence
Joint Authors
Arroyo, Rossana
Benchimol, Marlene
Rendón-Gandarilla, Francisco Javier
Ramón-Luing, Lucero de los Angeles
Ortega-López, Jaime
Rosa de Andrade, Ivone
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-01
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
The goal of this paper was to characterize a Trichomonas vaginalis cysteine proteinase (CP) legumain-1 (TvLEGU-1) and determine its potential role as a virulence factor during T.
vaginalis infection.
A 30-kDa band, which migrates in three protein spots (pI~6.3, ~6.5, and ~6.7) with a different type and level of phosphorylation, was identified as TvLEGU-1 by one- and two-dimensional Western blot (WB) assays, using a protease-rich trichomonad extract and polyclonal antibodies produced against the recombinant TvLEGU-1 (anti-TvLEGU-1r).
Its identification was confirmed by mass spectrometry.
Immunofluorescence, cell binding, and WB assays showed that TvLEGU-1 is upregulated by iron at the protein level, localized on the trichomonad surface and in lysosomes and Golgi complex, bound to the surface of HeLa cells, and was found in vaginal secretions.
Additionally, the IgG and Fab fractions of the anti-TvLEGU-1r antibody inhibited trichomonal cytoadherence up to 45%.
Moreover, the Aza-Peptidyl Michael Acceptor that inhibited legumain proteolytic activity in live parasites also reduced levels of trichomonal cytoadherence up to 80%.
In conclusion, our data show that the proteolytic activity of TvLEGU-1 is necessary for trichomonal adherence.
Thus, TvLEGU-1 is a novel virulence factor upregulated by iron.
This is the first report that a legumain-like CP plays a role in a pathogen cytoadherence.
American Psychological Association (APA)
Rendón-Gandarilla, Francisco Javier& Ramón-Luing, Lucero de los Angeles& Ortega-López, Jaime& Rosa de Andrade, Ivone& Benchimol, Marlene& Arroyo, Rossana. 2013. The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role in Trichomonas vaginalis Cytoadherence. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004587
Modern Language Association (MLA)
Rendón-Gandarilla, Francisco Javier…[et al.]. The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role in Trichomonas vaginalis Cytoadherence. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1004587
American Medical Association (AMA)
Rendón-Gandarilla, Francisco Javier& Ramón-Luing, Lucero de los Angeles& Ortega-López, Jaime& Rosa de Andrade, Ivone& Benchimol, Marlene& Arroyo, Rossana. The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role in Trichomonas vaginalis Cytoadherence. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004587