The Omega-3 Polyunsaturated Fatty Acid DHA Induces Simultaneous Apoptosis and Autophagy via Mitochondrial ROS-Mediated Akt-mTOR Signaling in Prostate Cancer Cells Expressing Mutant p53
Joint Authors
Shin, Soyeon
Jing, Kaipeng
Jeong, Soyeon
Kim, Nayeong
Song, Kyoung-Sub
Heo, Jun-Young
Park, Ji-Hoon
Seo, Kang-Sik
Han, Jeongsu
Park, Jong-Il
Kweon, Gi-Ryang
Park, Seung-Kiel
Wu, Tong
Hwang, Byung-Doo
Lim, Kyu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Docosahexaenoic acid (DHA) induces autophagy-associated apoptotic cell death in wild-type p53 cancer cells via regulation of p53.
The present study investigated the effects of DHA on PC3 and DU145 prostate cancer cell lines harboring mutant p53.
Results show that, in addition to apoptosis, DHA increased the expression levels of lipidated form LC3B and potently stimulated the autophagic flux, suggesting that DHA induces both autophagy and apoptosis in cancer cells expressing mutant p53.
DHA led to the generation of mitochondrial reactive oxygen species (ROS), as shown by the mitochondrial ROS-specific probe mitoSOX.
Similarly, pretreatment with the antioxidant N-acetyl-cysteine (NAC) markedly inhibited both the autophagy and the apoptosis triggered by DHA, indicating that mitochondrial ROS mediate the cytotoxicity of DHA in mutant p53 cells.
Further, DHA reduced the levels of phospho-Akt and phospho-mTOR in a concentration-dependent manner, while NAC almost completely blocked that effect.
Collectively, these findings present a novel mechanism of ROS-regulated apoptosis and autophagy that involves Akt-mTOR signaling in prostate cancer cells with mutant p53 exposed to DHA.
American Psychological Association (APA)
Shin, Soyeon& Jing, Kaipeng& Jeong, Soyeon& Kim, Nayeong& Song, Kyoung-Sub& Heo, Jun-Young…[et al.]. 2013. The Omega-3 Polyunsaturated Fatty Acid DHA Induces Simultaneous Apoptosis and Autophagy via Mitochondrial ROS-Mediated Akt-mTOR Signaling in Prostate Cancer Cells Expressing Mutant p53. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004609
Modern Language Association (MLA)
Shin, Soyeon…[et al.]. The Omega-3 Polyunsaturated Fatty Acid DHA Induces Simultaneous Apoptosis and Autophagy via Mitochondrial ROS-Mediated Akt-mTOR Signaling in Prostate Cancer Cells Expressing Mutant p53. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004609
American Medical Association (AMA)
Shin, Soyeon& Jing, Kaipeng& Jeong, Soyeon& Kim, Nayeong& Song, Kyoung-Sub& Heo, Jun-Young…[et al.]. The Omega-3 Polyunsaturated Fatty Acid DHA Induces Simultaneous Apoptosis and Autophagy via Mitochondrial ROS-Mediated Akt-mTOR Signaling in Prostate Cancer Cells Expressing Mutant p53. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004609
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004609