Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis
Joint Authors
Cao, Yunying
Zhang, Qian
Chen, Yanhong
Zhao, Hua
Lang, Youzhong
Yu, Chunmei
Yang, Jianchang
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
High temperature impedes the growth and productivity of various crop species.
To date, rice (Oryza sativa L.) has not been exploited to understand the molecular basis of its abnormally high level of temperature tolerance.
To identify transcripts induced by heat stress, twenty-day-old rice seedlings of different rice cultivars suffering from heat stress were treated at different times, and differential gene expression analyses in leaves were performed by cDNA-AFLP and further verified by real-time RT-PCR.
In aggregate, more than three thousand different fragments were indentified, and 49 fragments were selected for the sequence and differential expressed genes were classified functionally into different groups.
6 of 49 fragments were measured by real-time RT-PCR.
In addition, the variations of three different polyamine contents in response to heat stress through high-performance liquid chromatography (HPLC) analysis were also performed.
The results and their direct and indirect relationships to heat stress tolerance mechanism were discussed.
American Psychological Association (APA)
Cao, Yunying& Zhang, Qian& Chen, Yanhong& Zhao, Hua& Lang, Youzhong& Yu, Chunmei…[et al.]. 2013. Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004626
Modern Language Association (MLA)
Cao, Yunying…[et al.]. Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004626
American Medical Association (AMA)
Cao, Yunying& Zhang, Qian& Chen, Yanhong& Zhao, Hua& Lang, Youzhong& Yu, Chunmei…[et al.]. Identification of Differential Expression Genes in Leaves of Rice (Oryza sativa L.) in Response to Heat Stress by cDNA-AFLP Analysis. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004626