Purification and Characterization of Tannin Acyl Hydrolase Produced by Mixed Solid State Fermentation of Wheat Bran and Marigold Flower by Penicillium notatum NCIM 923
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-17
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Tannin acyl hydrolase produced extracellularly by the fungal strain Penicillium notatum NCIM 923 in mixed solid state fermentation of wheat bran and marigold flower in the ratio 4 : 1 was purified from the cell-free extract broth by ammonium sulphate fractionation followed by diethylaminoethyl-cellulose column chromatography.
Tannase was purified by 19.89-fold with yield of 11.77%.
The specific activity of crude tannase was found to be 1.31 U/mg protein while that of purified tannase was 22.48 U/mg protein.
SDS-PAGE analysis indicated that the enzyme is dimeric with one major band of molecular mass 97 kDa and a very light band of molecular mass 43 kDa.
Temperature of 35 to 40°C and pH 5 were optimum for tannase activity.
The enzyme retained more than 60% of its stability at 60°C and 40% stability at pH 3 and 8, respectively.
Km was found to be 0.33×10-2 M and Vmax=40 U/mg.
Since the enzyme is active over a wide range of pH and temperature, it could find potential use in the food processing industry.
American Psychological Association (APA)
Gayen, Saswati& Ghosh, Uma. 2013. Purification and Characterization of Tannin Acyl Hydrolase Produced by Mixed Solid State Fermentation of Wheat Bran and Marigold Flower by Penicillium notatum NCIM 923. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004688
Modern Language Association (MLA)
Gayen, Saswati& Ghosh, Uma. Purification and Characterization of Tannin Acyl Hydrolase Produced by Mixed Solid State Fermentation of Wheat Bran and Marigold Flower by Penicillium notatum NCIM 923. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1004688
American Medical Association (AMA)
Gayen, Saswati& Ghosh, Uma. Purification and Characterization of Tannin Acyl Hydrolase Produced by Mixed Solid State Fermentation of Wheat Bran and Marigold Flower by Penicillium notatum NCIM 923. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004688
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004688