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Deciphering the Intracellular Fate of Propionibacterium acnes in Macrophages
Joint Authors
Fischer, Natalie
Mak, Tim N.
Shinohara, Debika Biswal
Sfanos, Karen S.
Meyer, Thomas F.
Brüggemann, Holger
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Propionibacterium acnes is a Gram-positive bacterium that colonizes various niches of the human body, particularly the sebaceous follicles of the skin.
Over the last years a role of this common skin bacterium as an opportunistic pathogen has been explored.
Persistence of P.
acnes in host tissue has been associated with chronic inflammation and disease development, for example, in prostate pathologies.
This study investigated the intracellular fate of P.
acnes in macrophages after phagocytosis.
In a mouse model of P.
acnes-induced chronic prostatic inflammation, the bacterium could be detected in prostate-infiltrating macrophages at 2 weeks postinfection.
Further studies performed in the human macrophage cell line THP-1 revealed intracellular survival and persistence of P.
acnes but no intracellular replication or escape from the host cell.
Confocal analyses of phagosome acidification and maturation were performed.
Acidification of P.
acnes-containing phagosomes was observed at 6 h postinfection but then lost again, indicative of cytosolic escape of P.
acnes or intraphagosomal pH neutralization.
No colocalization with the lysosomal markers LAMP1 and cathepsin D was observed, implying that the P.
acnes-containing phagosome does not fuse with lysosomes.
Our findings give first insights into the intracellular fate of P.
acnes; its persistency is likely to be important for the development of P.
acnes-associated inflammatory diseases.
American Psychological Association (APA)
Fischer, Natalie& Mak, Tim N.& Shinohara, Debika Biswal& Sfanos, Karen S.& Meyer, Thomas F.& Brüggemann, Holger. 2013. Deciphering the Intracellular Fate of Propionibacterium acnes in Macrophages. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004700
Modern Language Association (MLA)
Fischer, Natalie…[et al.]. Deciphering the Intracellular Fate of Propionibacterium acnes in Macrophages. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004700
American Medical Association (AMA)
Fischer, Natalie& Mak, Tim N.& Shinohara, Debika Biswal& Sfanos, Karen S.& Meyer, Thomas F.& Brüggemann, Holger. Deciphering the Intracellular Fate of Propionibacterium acnes in Macrophages. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004700
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004700