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Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran
Joint Authors
Pakshir, Keyvan
Rahimi Ghiasi, Moosa
Zomorodian, Kamiar
Gharavi, Ali Reza
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Keratinophilic fungi are an important group of fungi that live in soil.
The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz.
Materials and Methods.
A total of 196 soil samples from 43 parks were collected.
Isolation of the fungi was performed by hair bait technique.
The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis.
ITS region of ribosomal DNA was amplified and the PCR products were sequenced.
Results.
411 isolates from 22 genera were identified.
Fusarium (23.8%), Chrysosporium (13.13%), Acremonium (12.65%), Penicillium (12.39%), Microsporum gypseum (1.94%), Bionectria ochroleuca (1.21%), Bipolaris spicifera (1.21%), Scedosporium apiospermum (0.82%), Phialophora reptans (0.82%), Cephalosporium curtipes (0.49%), Scedosporium dehoogii (0.24%), Ochroconis constricta (0.24%), Nectria mauritiicola (0.49%), Chaetomium (0.49%), Scopulariopsis (0.24%), Malbranchea (0.24%), and Tritirachium (0.24%) were the most important isolates.
Most of the fungi were isolated from the soils with the PH range of 7 to 8.
Conclusion.
Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi.
American Psychological Association (APA)
Pakshir, Keyvan& Rahimi Ghiasi, Moosa& Zomorodian, Kamiar& Gharavi, Ali Reza. 2013. Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1004733
Modern Language Association (MLA)
Pakshir, Keyvan…[et al.]. Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1004733
American Medical Association (AMA)
Pakshir, Keyvan& Rahimi Ghiasi, Moosa& Zomorodian, Kamiar& Gharavi, Ali Reza. Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1004733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004733