Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids
Joint Authors
Lee, Ju Hee
Shin, Jung U.
Jung, Inhee
Lee, Hemin
Rah, Dong Kyun
Jung, Jin Young
Lee, Won Jai
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis.
Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools.
Objective.
We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids.
Methods.
Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts.
Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE.
We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry.
Results.
Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts.
Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts.
Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF-β, and PCNA expressions in keloids compared to normal tissue.
Conclusion.
Hsp70 is overexpressed in keloid fibroblasts and tissue.
The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids.
American Psychological Association (APA)
Lee, Ju Hee& Shin, Jung U.& Jung, Inhee& Lee, Hemin& Rah, Dong Kyun& Jung, Jin Young…[et al.]. 2013. Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004737
Modern Language Association (MLA)
Lee, Ju Hee…[et al.]. Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004737
American Medical Association (AMA)
Lee, Ju Hee& Shin, Jung U.& Jung, Inhee& Lee, Hemin& Rah, Dong Kyun& Jung, Jin Young…[et al.]. Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004737