Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis
Joint Authors
Qian, Zhong
Zhao, Jingjing
Bai, Xue
Tong, Wei
Chen, Zhen
Wei, Hanfu
Wang, Quanhui
Liu, Siqi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In the genome of Thermoanaerobacter tengcongensis, three genes belonging to ROK (Repressor, ORF, and Kinase) family are annotated as glucokinases (GLKs).
Using enzyme assays, the three GLKs were identified as ATP-dependent GLK (ATP-GLK), ADP-dependent GLK (ADP-GLK), and N-acetyl-glucosamine/mannosamine kinase (glu/man-NacK).
The kinetic properties of the three GLKs such as Km, Vmax, optimal pH, and temperature were characterized, demonstrating that these enzymes performed the specific functions against varied substrates and under different temperatures.
The abundance of ATP-GLK was attenuated when culture temperature was elevated and was almost undetectable at 80°C, whereas the ADP-GLK abundance was insensitive to temperature changes.
Using degradation assays, ATP-GLK was found to have significantly faster degradation than ADP-GLK at 80°C.
Co-immunoprecipitation results revealed that heat shock protein 60 (HSP60) could interact with ATP-GLK and ADP-GLK at 60 and 75°C, whereas at 80°C, the interaction was only effectively with ADP-GLK but not ATP-GLK.
The functions of GLKs in T.
tengcongensis are temperature dependent, likely regulated through interactions with HSP60.
American Psychological Association (APA)
Qian, Zhong& Zhao, Jingjing& Bai, Xue& Tong, Wei& Chen, Zhen& Wei, Hanfu…[et al.]. 2013. Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004800
Modern Language Association (MLA)
Qian, Zhong…[et al.]. Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004800
American Medical Association (AMA)
Qian, Zhong& Zhao, Jingjing& Bai, Xue& Tong, Wei& Chen, Zhen& Wei, Hanfu…[et al.]. Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004800
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004800