Effect of Adjuvant Magnetic Fields in Radiotherapy on Non-Small-Cell Lung Cancer Cells In Vitro
Joint Authors
Feng, Jianguo
Sheng, Huaying
Zhu, Chihong
Jiang, Hao
Ma, Shenglin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
|Objectives.
To explore sensitization and possible mechanisms of adjuvant magnetic fields (MFs) in radiotherapy (RT) of non-small-cell lung cancer.
Methods.
Human A549 lung adenocarcinoma cells were treated with MF, RT, and combined MF-RT.
Colony-forming efficiency was calculated, cell cycle and apoptosis were measured, and changes in cell cycle- and apoptosis-related gene expression were measured by microarray.
Results.
A 0.5 T, 8 Hz stationary MF showed a duration-dependent inhibitory effect lasting for 1–4 hours.
The MF-treated groups had significantly greater cell inhibition than did controls (P<0.05).
Surviving fractions and growth curves derived from colony-forming assay showed that the MF-only, RT-only, and MF-RT groups had inhibited cell growth; the MF-RT group showed a synergetic effect.
Microarray of A549 cells exposed for 1 hour to MF showed that 19 cell cycle- and apoptosis-related genes had 2-fold upregulation and 40 genes had 2-fold downregulation.
MF significantly arrested cells in G2 and M phases, apparently sensitizing the cells to RT.
Conclusions.
MF may inhibit A549 cells and can increase their sensitivity to RT, possibly by affecting cell cycle- and apoptosis-related signaling pathways.
American Psychological Association (APA)
Feng, Jianguo& Sheng, Huaying& Zhu, Chihong& Jiang, Hao& Ma, Shenglin. 2013. Effect of Adjuvant Magnetic Fields in Radiotherapy on Non-Small-Cell Lung Cancer Cells In Vitro. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004816
Modern Language Association (MLA)
Feng, Jianguo…[et al.]. Effect of Adjuvant Magnetic Fields in Radiotherapy on Non-Small-Cell Lung Cancer Cells In Vitro. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1004816
American Medical Association (AMA)
Feng, Jianguo& Sheng, Huaying& Zhu, Chihong& Jiang, Hao& Ma, Shenglin. Effect of Adjuvant Magnetic Fields in Radiotherapy on Non-Small-Cell Lung Cancer Cells In Vitro. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004816