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“Zebrafishing” for Novel Genes Relevant to the Glomerular Filtration Barrier
Joint Authors
Schiffer, Mario
Hanke, Nils
Staggs, Lynne
Schroder, Patricia
Litteral, Jennifer
Fleig, Susanne
Kaufeld, Jessica
Pauli, Cornelius
Haller, Hermann
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Data for genes relevant to glomerular filtration barrier function or proteinuria is continually increasing in an era of microarrays, genome-wide association studies, and quantitative trait locus analysis.
Researchers are limited by published literature searches to select the most relevant genes to investigate.
High-throughput cell cultures and other in vitro systems ultimately need to demonstrate proof in an in vivo model.
Generating mammalian models for the genes of interest is costly and time intensive, and yields only a small number of test subjects.
These models also have many pitfalls such as possible embryonic mortality and failure to generate phenotypes or generate nonkidney specific phenotypes.
Here we describe an in vivo zebrafish model as a simple vertebrate screening system to identify genes relevant to glomerular filtration barrier function.
Using our technology, we are able to screen entirely novel genes in 4–6 weeks in hundreds of live test subjects at a fraction of the cost of a mammalian model.
Our system produces consistent and reliable evidence for gene relevance in glomerular kidney disease; the results then provide merit for further analysis in mammalian models.
American Psychological Association (APA)
Hanke, Nils& Staggs, Lynne& Schroder, Patricia& Litteral, Jennifer& Fleig, Susanne& Kaufeld, Jessica…[et al.]. 2013. “Zebrafishing” for Novel Genes Relevant to the Glomerular Filtration Barrier. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1004818
Modern Language Association (MLA)
Hanke, Nils…[et al.]. “Zebrafishing” for Novel Genes Relevant to the Glomerular Filtration Barrier. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1004818
American Medical Association (AMA)
Hanke, Nils& Staggs, Lynne& Schroder, Patricia& Litteral, Jennifer& Fleig, Susanne& Kaufeld, Jessica…[et al.]. “Zebrafishing” for Novel Genes Relevant to the Glomerular Filtration Barrier. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1004818
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004818