Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey
Joint Authors
Coşkun, Kübra Açıkalın
Özçelik, Semra
Tutar, Lütfi
Elaldı, Nazif
Tutar, Yusuf
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The present work focuses on a local survey of free-living amoebae (FLA) that cause opportunistic and nonopportunistic infections in humans.
Determining the prevalence of FLA in water sources can shine a light on the need to prevent FLA related illnesses.
A total of 150 samples of tap water were collected from six districts of Sivas province.
The samples were filtered and seeded on nonnutrient agar containing Escherichia coli spread.
Thirty-three (22%) out of 150 samples were found to be positive for FLA.
The FLA were identified by morphology and by PCR using 18S rDNA gene.
The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of three different species, Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis.
Naegleria fowleri, Balamuthia mandrillaris, or Sappinia sp.
was not isolated during the study.
All A.
castellanii and A.
polyphaga sequence types were found to be genotype T4 that contains most of the pathogenic Acanthamoeba strains.
The results indicated the occurrence and distribution of FLA species in tap water in these localities of Sivas, Turkey.
Furthermore, the presence of temperature tolerant Acanthamoeba genotype T4 in tap water in the region must be taken into account for health risks.
American Psychological Association (APA)
Coşkun, Kübra Açıkalın& Özçelik, Semra& Tutar, Lütfi & Elaldı, Nazif& Tutar, Yusuf. 2013. Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004827
Modern Language Association (MLA)
Coşkun, Kübra Açıkalın…[et al.]. Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004827
American Medical Association (AMA)
Coşkun, Kübra Açıkalın& Özçelik, Semra& Tutar, Lütfi & Elaldı, Nazif& Tutar, Yusuf. Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004827