N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA
Joint Authors
Sakudo, Akikazu
Shimizu, Naohiro
Imanishi, Yuichiro
Ikuta, Kazuyoshi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We have recently treated with N2 gas plasma and achieved inactivation of bacteria.
However, the effect of N2 gas plasma on viruses remains unclear.
With the aim of developing this technique, we analyzed the virucidal effect of N2 gas plasma on influenza virus and its influence on the viral components.
We treated influenza virus particles with inert N2 gas plasma (1.5 kpps; kilo pulses per second) produced by a short high-voltage pulse generated from a static induction thyristor power supply.
A bioassay using chicken embryonated eggs demonstrated that N2 gas plasma inactivated influenza virus in allantoic fluid within 5 min.
Immunochromatography, enzyme-linked immunosorbent assay, and Coomassie brilliant blue staining showed that N2 gas plasma treatment of influenza A and B viruses in nasal aspirates and allantoic fluids as well as purified influenza A and B viruses induced degradation of viral proteins including nucleoprotein.
Analysis using the polymerase chain reaction suggested that N2 gas plasma treatment induced changes in the viral RNA genome.
Scanning electron microscopy analysis showed that aggregation and fusion of influenza viruses were induced by N2 gas plasma treatment.
We believe these biochemical changes may contribute to the inactivation of influenza viruses by N2 gas plasma.
American Psychological Association (APA)
Sakudo, Akikazu& Shimizu, Naohiro& Imanishi, Yuichiro& Ikuta, Kazuyoshi. 2013. N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004889
Modern Language Association (MLA)
Sakudo, Akikazu…[et al.]. N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004889
American Medical Association (AMA)
Sakudo, Akikazu& Shimizu, Naohiro& Imanishi, Yuichiro& Ikuta, Kazuyoshi. N2 Gas Plasma Inactivates Influenza Virus by Inducing Changes in Viral Surface Morphology, Protein, and Genomic RNA. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004889
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004889