Is the Second Sodium Pump Electrogenic?
Joint Authors
Thomas, L. E.
Rocafull, M. A.
del Castillo, J. R.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Transepithelial sodium transport is a process that involves active Na+ transport at the basolateral membrane of the epithelial cell.
This process is mediated by the Na+/K+ pump, which exchanges 3 internal Na+ by 2 external K+ inducing a net charge movement and the second Na+ pump, which transports Na+ accompanied by Cl− and water.
It has been suggested that this pump could also be electrogenic.
Herein, we evaluated, in MDCK cells, the short-circuit current (Isc) generated by these Na+ pumps at the basolateral membrane of the epithelial cells, using amphotericin B as an apical permeabilizing agent.
In Cl−-containing media, Isc induced by amphotericin B is totally inhibited by ouabain, indicating that only the electrogenic Na+/K+ pump is detectable in the presence of Cl−.
Electrogenicity of the second Na+ pump can be demonstrated in Cl−-free media.
The existence of a furosemide-sensitive component of Isc, in addition to an ouabain-sensitive one, was identified in absence of chloride.
Passive Cl− movement associated with the function of the second Na+ pump seems to be regulated by the pump itself.
These results demonstrate that the second Na+ pump is an electroneutral mechanism result from the stoichiometric movement of Na+ and Cl− across the basolateral plasma membrane of the epithelial cell.
American Psychological Association (APA)
Thomas, L. E.& Rocafull, M. A.& del Castillo, J. R.. 2012. Is the Second Sodium Pump Electrogenic?. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004905
Modern Language Association (MLA)
Thomas, L. E.…[et al.]. Is the Second Sodium Pump Electrogenic?. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004905
American Medical Association (AMA)
Thomas, L. E.& Rocafull, M. A.& del Castillo, J. R.. Is the Second Sodium Pump Electrogenic?. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004905