Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
Joint Authors
Yang, Chao-Hsun
Kuo, Wan-Ting
Chuang, Yun-Ting
Chen, Cheng-Yu
Lin, Chih-Chien
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle.
Cyclins are degraded by the ubiquitin-proteasome system during cell cycle.
Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required.
Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins.
Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins.
Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future.
American Psychological Association (APA)
Yang, Chao-Hsun& Kuo, Wan-Ting& Chuang, Yun-Ting& Chen, Cheng-Yu& Lin, Chih-Chien. 2013. Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004987
Modern Language Association (MLA)
Yang, Chao-Hsun…[et al.]. Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1004987
American Medical Association (AMA)
Yang, Chao-Hsun& Kuo, Wan-Ting& Chuang, Yun-Ting& Chen, Cheng-Yu& Lin, Chih-Chien. Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1004987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004987