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The CULTEX RFS: A Comprehensive Technical Approach for the In Vitro Exposure of Airway Epithelial Cells to the Particulate Matter at the Air-Liquid Interface
Joint Authors
Aufderheide, Michaela
Halter, Beat
Möhle, Niklas
Hochrainer, Dieter
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The EU Regulation on Registration, Evaluation, Authorization and Restriction of Chemicals (REACH) demands the implementation of alternative methods for analyzing the hazardous effects of chemicals including particulate formulations.
In the field of inhalation toxicology, a variety of in vitro models have been developed for such studies.
To simulate the in vivo situation, an adequate exposure device is necessary for the direct exposure of cultivated lung cells at the air-liquid interface (ALI).
The CULTEX RFS fulfills these requirements and has been optimized for the exposure of cells to atomized suspensions, gases, and volatile compounds as well as micro- and nanosized particles.
This study provides information on the construction and functional aspects of the exposure device.
By using the Computational Fluid Dynamics (CFD) analysis, the technical design was optimized to realize a stable, reproducible, and homogeneous deposition of particles.
The efficiency of the exposure procedure is demonstrated by exposing A549 cells dose dependently to lactose monohydrate, copper(II) sulfate, copper(II) oxide, and micro- and nanoparticles.
All copper compounds induced cytotoxic effects, most pronounced for soluble copper(II) sulfate.
Micro- and nanosized copper(II) oxide also showed a dose-dependent decrease in the cell viability, whereby the nanosized particles decreased the metabolic activity of the cells more severely.
American Psychological Association (APA)
Aufderheide, Michaela& Halter, Beat& Möhle, Niklas& Hochrainer, Dieter. 2013. The CULTEX RFS: A Comprehensive Technical Approach for the In Vitro Exposure of Airway Epithelial Cells to the Particulate Matter at the Air-Liquid Interface. BioMed Research International،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1004989
Modern Language Association (MLA)
Aufderheide, Michaela…[et al.]. The CULTEX RFS: A Comprehensive Technical Approach for the In Vitro Exposure of Airway Epithelial Cells to the Particulate Matter at the Air-Liquid Interface. BioMed Research International No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-1004989
American Medical Association (AMA)
Aufderheide, Michaela& Halter, Beat& Möhle, Niklas& Hochrainer, Dieter. The CULTEX RFS: A Comprehensive Technical Approach for the In Vitro Exposure of Airway Epithelial Cells to the Particulate Matter at the Air-Liquid Interface. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-1004989
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004989