Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons
Joint Authors
Brown, Amanda L.
Day, Trevor A.
Dayas, Christopher V.
Smith, Doug W.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells.
However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample.
Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity.
The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold.
For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively.
Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes.
Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples.
Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups.
This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity.
Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.
American Psychological Association (APA)
Brown, Amanda L.& Day, Trevor A.& Dayas, Christopher V.& Smith, Doug W.. 2013. Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005023
Modern Language Association (MLA)
Brown, Amanda L.…[et al.]. Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005023
American Medical Association (AMA)
Brown, Amanda L.& Day, Trevor A.& Dayas, Christopher V.& Smith, Doug W.. Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005023
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005023