Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
Joint Authors
Paul, Debasish
Kumar, Avinash
Gajbhiye, Akshada
Santra, Manas K.
Srikanth, Rapole
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-17
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Accurate diagnosis and proper monitoring of cancer patients remain a key obstacle for successful cancer treatment and prevention.
Therein comes the need for biomarker discovery, which is crucial to the current oncological and other clinical practices having the potential to impact the diagnosis and prognosis.
In fact, most of the biomarkers have been discovered utilizing the proteomics-based approaches.
Although high-throughput mass spectrometry-based proteomic approaches like SILAC, 2D-DIGE, and iTRAQ are filling up the pitfalls of the conventional techniques, still serum proteomics importunately poses hurdle in overcoming a wide range of protein concentrations, and also the availability of patient tissue samples is a limitation for the biomarker discovery.
Thus, researchers have looked for alternatives, and profiling of candidate biomarkers through tissue culture of tumor cell lines comes up as a promising option.
It is a rich source of tumor cell-derived proteins, thereby, representing a wide array of potential biomarkers.
Interestingly, most of the clinical biomarkers in use today (CA 125, CA 15.3, CA 19.9, and PSA) were discovered through tissue culture-based system and tissue extracts.
This paper tries to emphasize the tissue culture-based discovery of candidate biomarkers through various mass spectrometry-based proteomic approaches.
American Psychological Association (APA)
Paul, Debasish& Kumar, Avinash& Gajbhiye, Akshada& Santra, Manas K.& Srikanth, Rapole. 2013. Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture. BioMed Research International،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-1005099
Modern Language Association (MLA)
Paul, Debasish…[et al.]. Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture. BioMed Research International No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-1005099
American Medical Association (AMA)
Paul, Debasish& Kumar, Avinash& Gajbhiye, Akshada& Santra, Manas K.& Srikanth, Rapole. Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-1005099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005099