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Cytotoxic Effect of Ethanol Extract of Microalga, Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line
Joint Authors
Alitheen, Noorjahan Banu Mohamed
Ebrahimi Nigjeh, Siyamak
Yusoff, Fatimah Md
Rasoli, Mehdi
Omar, Abdul Rahman
Yeap, Swee Keong
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Marine microalgae have been prominently featured in cancer research.
Here, we examined cytotoxic effect and apoptosis mechanism of crude ethanol extracts of an indigenous microalga, Chaetoceros calcitrans (UPMAAHU10) on human breast cell lines.
MCF-7 was more sensitive than MCF-10A with IC50 value of 3.00±0.65, whilst the IC50 value of Tamoxifen against MCF-7 was 12.00±0.52 μg/mL after 24 hour incubation.
Based on Annexin V/Propidium iodide and cell cycle flow cytometry analysis, it was found that inhibition of cell growth by EEC on MCF-7 cells was through the induction of apoptosis without cell cycle arrest.
The apoptotic cells at subG0/G1 phase in treated MCF-7 cells at 48 and 72 hours showed 34 and 16 folds increased compared to extract treated MCF-10A cells which showed only 6 and 7 folds increased at the same time points, respectively.
Based on GeXP study, EEC induced apoptosis on MCF-7 cells via modulation of CDK2, MDM2, p21Cip1, Cyclin A2, Bax and Bcl-2.
The EEC treated MCF-7 cells also showed an increase in Bax/Bcl-2 ratio that in turn activated the caspase-dependent pathways by activating caspase 7.
Thus, marine microalga, Chaetoceros calcitrans may be considered a good candidate to be developed as a new anti-breast cancer drug.
American Psychological Association (APA)
Ebrahimi Nigjeh, Siyamak& Yusoff, Fatimah Md& Alitheen, Noorjahan Banu Mohamed& Rasoli, Mehdi& Yeap, Swee Keong& Omar, Abdul Rahman. 2012. Cytotoxic Effect of Ethanol Extract of Microalga, Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005103
Modern Language Association (MLA)
Ebrahimi Nigjeh, Siyamak…[et al.]. Cytotoxic Effect of Ethanol Extract of Microalga, Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005103
American Medical Association (AMA)
Ebrahimi Nigjeh, Siyamak& Yusoff, Fatimah Md& Alitheen, Noorjahan Banu Mohamed& Rasoli, Mehdi& Yeap, Swee Keong& Omar, Abdul Rahman. Cytotoxic Effect of Ethanol Extract of Microalga, Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005103
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005103