Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry
Joint Authors
Pandey, Gouri Shankar
Tseng, Sandra C.
Howard, Tom E.
Sauna, Zuben E.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Flow cytometry is widely used in cancer research for diagnosis, detection of minimal residual disease, as well as immune monitoring and profiling following immunotherapy.
Detection of specific host proteins for diagnosis predominantly uses quantitative PCR and western blotting assays.
In this study, we optimized a flow cytometry-based detection assay for Factor VIII protein in peripheral blood mononuclear cells (PBMCs).
An indirect intracellular staining (ICS) method was standardized using monoclonal antibodies to different domains of human Factor VIII protein.
The FVIII protein expression level was estimated by calculating the mean and median fluorescence intensities (MFI) values for each monoclonal antibody.
ICS staining of transiently transfected cell lines supported the method's specificity.
Intracellular FVIII protein expression was also detected by the monoclonal antibodies used in the study in PBMCs of five blood donors.
In summary, our data suggest that intracellular FVIII detection in PBMCs of hemophilia A patients can be a rapid and reliable method to detect intracellular FVIII levels.
American Psychological Association (APA)
Pandey, Gouri Shankar& Tseng, Sandra C.& Howard, Tom E.& Sauna, Zuben E.. 2013. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005127
Modern Language Association (MLA)
Pandey, Gouri Shankar…[et al.]. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005127
American Medical Association (AMA)
Pandey, Gouri Shankar& Tseng, Sandra C.& Howard, Tom E.& Sauna, Zuben E.. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005127
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005127