Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events
Joint Authors
Liu, Jing
Xie, Wanqin
Lange, Miles D.
Hong, Sang Yong
Su, Kaihong
Zhang, Zhixin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
It has been recognized for a long time that engagement of B cell antigen receptors (BCRs) on immature B cells or mature B cells leads to completely opposite cell fate decisions.
The underlying mechanism remains unclear.
Here, we show that crosslinking of BCRs on human EU12 μHC+ immature B cells resulted in complete internalization of cell surface BCRs.
After loss of cell surface BCRs, restimulation of EU12 μHC+ cells showed impaired Ca2+ flux, delayed SYK phosphorylation, and decreased CD19 and FOXO1 phosphorylation, which differ from those in mature Daudi or Ramos B cells with partial internalization of BCRs.
In contrast, sustained phosphorylation and reactivation of ERK upon restimulation were observed in the EU12 μHC+ cells after BCR internalization.
Taken together, these results show that complete internalization of cell surface BCRs in EU12 μHC+ cells specifically alters the downstream signaling events, which may favor receptor editing versus cell activation.
American Psychological Association (APA)
Liu, Jing& Xie, Wanqin& Lange, Miles D.& Hong, Sang Yong& Su, Kaihong& Zhang, Zhixin. 2013. Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005165
Modern Language Association (MLA)
Liu, Jing…[et al.]. Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1005165
American Medical Association (AMA)
Liu, Jing& Xie, Wanqin& Lange, Miles D.& Hong, Sang Yong& Su, Kaihong& Zhang, Zhixin. Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005165