Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy
Joint Authors
Beltran, Nohra E.
Garcia, Laura E.
Garcia-Lorenzana, Mario
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The gastric mucosa ischemic tissular damage plays an important role in critical care patients’ outcome, because it is the first damaged tissue by compensatory mechanism during shock.
The aim of the study is to relate bioimpedance changes with tissular damage level generated by ischemia by means of confocal endomicroscopy and light microscopy.
Bioimpedance of the gastric mucosa and confocal images were obtained from Wistar male rats during basal and ischemia conditions.
They were anesthetized, and stain was applied (fluorescein and/or acriflavine).
The impedance spectroscopy catheter was inserted and then confocal endomicroscopy probe.
After basal measurements and biopsy, hepatic and gastric arteries clamping induced ischemia.
Finally, pyloric antrum tissue was preserved in buffered formaldehyde (10%) for histology processing using light microscopy.
Confocal images were equalized, binarized, and boundary defined, and infiltrations were quantified.
Impedance and infiltrations increased with ischemia showing significant changes between basal and ischemia conditions (P<0.01).
Light microscopy analysis allows detection of general alterations in cellular and tissular integrity, confirming gastric reactance and confocal images quantification increments obtained during ischemia.
American Psychological Association (APA)
Beltran, Nohra E.& Garcia, Laura E.& Garcia-Lorenzana, Mario. 2013. Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005204
Modern Language Association (MLA)
Beltran, Nohra E.…[et al.]. Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1005204
American Medical Association (AMA)
Beltran, Nohra E.& Garcia, Laura E.& Garcia-Lorenzana, Mario. Gastric Tissue Damage Analysis Generated by Ischemia: Bioimpedance, Confocal Endomicroscopy, and Light Microscopy. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1005204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005204