The Fold Variant BM4 Is Beneficial in a Therapeutic Bet v 1 Mouse Model

Abstract EN

Specific immunotherapy using recombinant allergens is clinically effective; still wild-type allergens can provoke treatment-induced side effects and often show poor immunogenicity in vivo.

Thus, we tested the low IgE-binding, highly immunogenic fold variant BM4 in a Bet v 1 mouse model.

Methods.

Recombinant BM4 was used as active vaccine ingredient to treat mice sensitized to Bet v 1.

As controls, mice were treated with either Bet v 1 or sham, and the humoral as well as cellular immune response was monitored.

Moreover, lung function and lung inflammation were analysed.

Results.

BM4 was more effective than wild-type Bet v 1 in inducing Bet v 1-specific blocking antibodies as well as IFN-γ and IL-10 producing T cells.

Further, birch pollen induced lung inflammation could be ameliorated significantly by BM4 treatment as demonstrated by a reduction of airway hyperresponsiveness and drastically decreased eosinophil counts in bronchoalveolar lavage fluids.

Conclusion.

The study outlines the high potential of BM4 as vaccine candidate for the treatment of Bet v 1-mediated birch pollen allergies.