High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties
Joint Authors
Li, Wei
Gao, Xiang
Ren, Junle
An, Ting
Liu, Yan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The cleaved amino-terminal fragment of human amyloid precursor protein (N-APP) binds death receptor 6 (DR6) and triggers a caspase-dependent self-destruction process, which was suggested to contribute to Alzheimer’s disease.
To investigate the N-APP-DR6-induced degeneration pathway at the molecular level, obtaining abundant and purified N-APP is fundamental and critical.
The recombinant N-APP has been produced in mammalian expression system.
However, the cost and yield disadvantages of mammalian expression system make it less ideal for protein mass production.
Here, we successfully expressed and purified recombinant N-terminal 18-285 amino acid residues of human amyloid precursor protein from the methylotrophic yeast Pichia pastoris with a high yield of 50 mg/L.
Flow cytometry indicated the purified N-APP-induced obvious apoptosis of human neuroblastoma SHEP cells.
American Psychological Association (APA)
Li, Wei& Gao, Xiang& Ren, Junle& An, Ting& Liu, Yan. 2013. High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005227
Modern Language Association (MLA)
Li, Wei…[et al.]. High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1005227
American Medical Association (AMA)
Li, Wei& Gao, Xiang& Ren, Junle& An, Ting& Liu, Yan. High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1005227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005227