Optimization of Dairy Sludge for Growth of Rhizobium Cells
Joint Authors
Singh, Ashok Kumar
Singh, Gauri
Gautam, Digvijay
Bedi, Manjinder Kaur
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-09
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
In this study dairy sludge was evaluated as an alternative cultivation medium for Rhizobium.
Growth of bacterial strains at different concentrations of Dairy sludge was monitored.
Maximum growth of all strains was observed at 60% Dairy sludge concentration.
At 60% optical density (OD) values are 0.804 for Rhizobium trifolii (MTCC905), 0.825 for Rhizobium trifolii (MTCC906), and 0.793 for Rhizobium meliloti (MTCC100).
Growth pattern of strains was observed at 60% Dairy sludge along with different synthetic media (tryptone yeast, Rhizobium minimal medium and yeast extract mannitol).
Growth in 60% Dairy sludge was found to be superior to standard media used for Rhizobium.
Media were optimized using 60% dairy sludge along with different concentrations of yeast extract (1–7 g/L) and mannitol (7–13 g/L) in terms of optical density at different time intervals, that is, 24, 48 and 72 hours.
Maximum growth was observed in 6 g/L of yeast extract and 12 g/L of mannitol at 48-hour incubation period in all strains.
The important environmental parameters such as pH were optimized using 60% dairy sludge, 60% dairy sludge +6 g/L yeast extract, and 60% dairy sludge +12 g/L mannitol.
The maximum growth of all strains was found at pH 7.0.
The present study recommends the use of 60% dairy sludge as a suitable growth medum for inoculant production.
American Psychological Association (APA)
Singh, Ashok Kumar& Singh, Gauri& Gautam, Digvijay& Bedi, Manjinder Kaur. 2013. Optimization of Dairy Sludge for Growth of Rhizobium Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1005248
Modern Language Association (MLA)
Singh, Ashok Kumar…[et al.]. Optimization of Dairy Sludge for Growth of Rhizobium Cells. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1005248
American Medical Association (AMA)
Singh, Ashok Kumar& Singh, Gauri& Gautam, Digvijay& Bedi, Manjinder Kaur. Optimization of Dairy Sludge for Growth of Rhizobium Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1005248
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005248