Correlation of N-Acetyltransferase 2 Genotype with Isoniazid Acetylation in Polish Tuberculosis Patients
Joint Authors
Augustynowicz-Kopeć, Ewa
Zabost, Anna
Brzezińska, Sylwia
Kozińska, Monika
Błachnio, Maria
Jagodziński, Jacek
Zwolska, Zofia
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Isoniazid (INH), a key agent in the treatment of tuberculosis (TB), is metabolized primarily by the genetically polymorphic N-acetyltransferase 2 (NAT2) enzyme.
Patients treated with INH can be classified as fast, intermediate, and slow acetylators.
The objective of this study was to explore the relationship between NAT2 genotypes and the serum concentrations of INH.
Blood samples from 130 patients were taken for the analysis, and plasma INH concentrations were determined by using the high-performance liquid chromatography (HPLC) technology.
Acetylation genotype was determined on genomic DNA by using an allele-specific polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) assay.
Once the NAT2 genotypes were established, patients were classified into three categories: fast, intermediate, and slow acetylators.
Of the 130 patients studied, 84 (64.6%) were slow, 39 (30%) were intermediate, and 7 (5.4%) were fast acetylators.
Analysis of INH concentrations in the blood of patients receiving the approximate doses of the drug revealed that, at the time intervals examined, the average concentration of INH was 2- to 7-fold higher among slow acetylators compared to fast and intermediate acetylators.
Conclusion.
Determining mutations in the NAT2 gene enabled the identification of the INH acetylation type in patients and the genotyping results were consistent with the phenotype determined by methods of measurement of drug bioavailability.
American Psychological Association (APA)
Zabost, Anna& Brzezińska, Sylwia& Kozińska, Monika& Błachnio, Maria& Jagodziński, Jacek& Zwolska, Zofia…[et al.]. 2013. Correlation of N-Acetyltransferase 2 Genotype with Isoniazid Acetylation in Polish Tuberculosis Patients. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1005279
Modern Language Association (MLA)
Zabost, Anna…[et al.]. Correlation of N-Acetyltransferase 2 Genotype with Isoniazid Acetylation in Polish Tuberculosis Patients. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1005279
American Medical Association (AMA)
Zabost, Anna& Brzezińska, Sylwia& Kozińska, Monika& Błachnio, Maria& Jagodziński, Jacek& Zwolska, Zofia…[et al.]. Correlation of N-Acetyltransferase 2 Genotype with Isoniazid Acetylation in Polish Tuberculosis Patients. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1005279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005279