Screening of a Protein That Interacts with the Matrix Attachment Region-Binding Protein from Dunaliella salina

Abstract EN

We isolated the matrix attachment region-binding protein (MBP) DMBP-1 from Dunaliella salina in our previous studies.

MBPs are part of the cis-acting protein family cluster.

The regulatory function possibly works through the interaction of the MBPs with each other.

In the present study, DMBP-1 was used as the bait in screening the D.

salina cDNA library for DMBP-1 interactors that could potentially mediate the DMBP-1-regulated functions.

A novel MBP, namely, DMBP-2, was identified as a DMBP-1 binding partner.

The cDNA of DMBP-1 was 823 bp long and contained a 573 bp open reading frame, which encoded a polypeptide of 191 amino acids.

The interaction between DMBP-2 and DMBP-1 was further confirmed through glutathione S-transferase pull-down assays.