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MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP
Joint Authors
Liu, Kuijie
Zhao, Hua
Yao, Hongliang
Lei, Sanlin
Lei, Zhendong
Li, Tiegang
Qi, Haizhi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
MicroRNAs are a class of small, noncoding RNAs that function as critical regulators of gene expression by targeting mRNAs for translational repression or degradation.
In this study, we demonstrate that expression of microRNA-124 (miR-124) is significantly downregulated in colorectal cancer tissues and cell lines, compared to the matched adjacent tissues.
We identified and confirmed inhibitor of apoptosis-stimulating protein of p53 (iASPP) as a novel, direct target of miR-124 using target prediction algorithms and luciferase reporter gene assays.
Overexpression of miR-124 suppressed iASPP protein expression, upregulated expression of the downstream signaling molecule nuclear factor-kappa B (NF-κB), and attenuated cell viability, proliferation, and colony formation in SW480 and HT-29 colorectal cancer cells in vitro.
Forced overexpression of iASPP partly rescued the inhibitory effect of miR-124 on SW480 and HT29 cell proliferation.
Taken together, these findings shed light on the role and mechanism of action of miR-124, indicate that the miR-124/iASPP axis can regulate the proliferation of colorectal cancer cells, and suggest that miR-124 may serve as a potential therapeutic target for colorectal cancer.
American Psychological Association (APA)
Liu, Kuijie& Zhao, Hua& Yao, Hongliang& Lei, Sanlin& Lei, Zhendong& Li, Tiegang…[et al.]. 2013. MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005307
Modern Language Association (MLA)
Liu, Kuijie…[et al.]. MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005307
American Medical Association (AMA)
Liu, Kuijie& Zhao, Hua& Yao, Hongliang& Lei, Sanlin& Lei, Zhendong& Li, Tiegang…[et al.]. MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005307
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005307