Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
Joint Authors
Tan, Liping
Zhang, Xinheng
Zheng, Guochao
Liu, Yuanjia
Li, Guoqing
Lin, Liqin
Hu, Wei
Lu, Pengyun
Alsarakibi, Muhamd
Luo, Qin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world.
A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A.
caninum in cats in Guangzhou, southern China.
Of the 102 fecal samples examined, the prevalence of A.
caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively.
Among them, the prevalence of single hookworm infection with A.
caninum was 54.90%, while mixed infections with both A.
caninum and A.
ceylanicum were 40.20%.
Comparative analysis of three complete ITS sequences obtained from cat-derived A.
caninum showed the same length (738 bp) as that of dog-derived A.
caninum.
However, the sequence variation range was 98.6%–100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A.
caninum isolates (AM850106, EU159416) in the same studied area.
The phylogenetic tree revealed A.
caninum derived from both cats and dogs in single cluster.
Results suggest that cats could be the main host of A.
caninum in China, which may cause cross-infection between dogs and cats in the same area.
American Psychological Association (APA)
Liu, Yuanjia& Zheng, Guochao& Alsarakibi, Muhamd& Zhang, Xinheng& Hu, Wei& Lu, Pengyun…[et al.]. 2013. Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005309
Modern Language Association (MLA)
Liu, Yuanjia…[et al.]. Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1005309
American Medical Association (AMA)
Liu, Yuanjia& Zheng, Guochao& Alsarakibi, Muhamd& Zhang, Xinheng& Hu, Wei& Lu, Pengyun…[et al.]. Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005309