Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status
Joint Authors
Schreier, Christina
Kremer, Werner
Huber, Fritz
Neumann, Sindy
Pagel, Philipp
Lienemann, Kai
Pestel, Sabine
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-23
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Spectroscopic analysis of urine samples from laboratory animals can be used to predict the efficacy and side effects of drugs.
This employs methods combining 1H NMR spectroscopy with quantification of biomarkers or with multivariate data analysis.
The most critical steps in data evaluation are analytical reproducibility of NMR data (collection, storage, and processing) and the health status of the animals, which may influence urine pH and osmolarity.
Methods.
We treated rats with a solvent, a diuretic, or a nephrotoxicant and collected urine samples.
Samples were titrated to pH 3 to 9, or salt concentrations increased up to 20-fold.
The effects of storage conditions and freeze-thaw cycles were monitored.
Selected metabolites and multivariate data analysis were evaluated after 1H NMR spectroscopy.
Results.
We showed that variation of pH from 3 to 9 and increases in osmolarity up to 6-fold had no effect on the quantification of the metabolites or on multivariate data analysis.
Storage led to changes after 14 days at 4°C or after 12 months at −20°C, independent of sample composition.
Multiple freeze-thaw cycles did not affect data analysis.
Conclusion.
Reproducibility of NMR measurements is not dependent on sample composition under physiological or pathological conditions.
American Psychological Association (APA)
Schreier, Christina& Kremer, Werner& Huber, Fritz& Neumann, Sindy& Pagel, Philipp& Lienemann, Kai…[et al.]. 2013. Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status. BioMed Research International،Vol. 2013, no. 2013, pp.1-19.
https://search.emarefa.net/detail/BIM-1005331
Modern Language Association (MLA)
Schreier, Christina…[et al.]. Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status. BioMed Research International No. 2013 (2013), pp.1-19.
https://search.emarefa.net/detail/BIM-1005331
American Medical Association (AMA)
Schreier, Christina& Kremer, Werner& Huber, Fritz& Neumann, Sindy& Pagel, Philipp& Lienemann, Kai…[et al.]. Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-19.
https://search.emarefa.net/detail/BIM-1005331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005331