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Proteinase-Activated Receptor-2 Agonist Activates Anti-Influenza Mechanisms and Modulates IFNγ-Induced Antiviral Pathways in Human Neutrophils
Joint Authors
Steinhoff, Martin
Feld, Micha
Shpacovitch, Victoria
Ehrhardt, Christina
Fastrich, Michaela
Goerge, Tobias
Ludwig, Stephan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Proteinase-activated receptor-2 (PAR2) is expressed by human leukocytes and participates in the development of inflammatory diseases.
Recent studies demonstrated an ability of PAR2 agonist to enhance IFNγ-induced antiviral responses of human leukocytes.
However, the precise cellular antiviral defense mechanisms triggered in leukocytes after stimulation with IFNγ and/or PAR2 agonist remain elusive.
Therefore, we aimed to identify neutrophil defense mechanisms involved in antiviral resistance.
Here we demonstrated that PAR2 agonist enhanced IFNγ-related reduction of influenza A virus (IAV) replication in human neutrophils.
PAR2-mediated decrease in IAV replication was associated with reduced NS-1 transcription.
Moreover, PAR2-dependent neutrophil activation resulted in enhanced myeloperoxidase degranulation and extracellular myeloperoxidase disrupted IAV.
The production of ROS was elevated in response to PAR2 activation.
Interestingly, IFNγ did not influence both effects: PAR2 agonist-triggered myeloperoxidase (MPO) release and reactive oxygen species (ROS) production, which are known to limit IAV infections.
In contrast, orthomyxovirus resistance gene A (MxA) protein expression was synergistically elevated through PAR2 agonist and IFNγ in neutrophils.
Altogether, these findings emphasize two PAR2-controlled antiviral mechanisms that are independent of or modulated by IFNγ.
American Psychological Association (APA)
Feld, Micha& Shpacovitch, Victoria& Ehrhardt, Christina& Fastrich, Michaela& Goerge, Tobias& Ludwig, Stephan…[et al.]. 2013. Proteinase-Activated Receptor-2 Agonist Activates Anti-Influenza Mechanisms and Modulates IFNγ-Induced Antiviral Pathways in Human Neutrophils. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005334
Modern Language Association (MLA)
Feld, Micha…[et al.]. Proteinase-Activated Receptor-2 Agonist Activates Anti-Influenza Mechanisms and Modulates IFNγ-Induced Antiviral Pathways in Human Neutrophils. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005334
American Medical Association (AMA)
Feld, Micha& Shpacovitch, Victoria& Ehrhardt, Christina& Fastrich, Michaela& Goerge, Tobias& Ludwig, Stephan…[et al.]. Proteinase-Activated Receptor-2 Agonist Activates Anti-Influenza Mechanisms and Modulates IFNγ-Induced Antiviral Pathways in Human Neutrophils. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005334
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005334