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Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells
Joint Authors
Ülker, Hayriye Esra
Ülker, Mustafa
Gümüş, Hasan Önder
Yalçın, Muhammet
Şengün, Abdulkadir
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements.
For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h.
The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells.
The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively.
Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P<0.05).
The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively.
None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P>0.05).
Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.
American Psychological Association (APA)
Ülker, Hayriye Esra& Ülker, Mustafa& Gümüş, Hasan Önder& Yalçın, Muhammet& Şengün, Abdulkadir. 2013. Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005389
Modern Language Association (MLA)
Ülker, Hayriye Esra…[et al.]. Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1005389
American Medical Association (AMA)
Ülker, Hayriye Esra& Ülker, Mustafa& Gümüş, Hasan Önder& Yalçın, Muhammet& Şengün, Abdulkadir. Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1005389
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005389