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Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells
Joint Authors
Pacchiarotti, Jason
Ramos, Thomas
Howerton, Kyle
Greilach, Scott
Zaragoza, Karina
Olmstead, Marnie
Izadyar, Fariborz
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Recent work in preservation of female fertility as well as new information on the nature of spermatogonial stem cells has prompted an investigation into the possibility of an effective clinical-grade procedure for the cryopreservation of testicular cells and/or tissue.
Clinical-grade reagents, validated equipment, and protocols consistent with cGTP/cGMP standards were used in developing a procedure suitable for the safe and effective cryopreservation of human testicular cells and tissues.
These procedures were designed to be compliant with the relevant FDA regulations.
The procedure proved to effectively cryopreserve both testicular cells and tissue.
The cryopreservation of testicular tissue was comparable in most aspects we measured to the cryopreservation of isolated cells, except that the viability of the cells from cryopreserved testicular tissue was found to be significantly higher.
On the other hand, cryopreservation of cells is preferred for cell analysis, quality control, and sterility testing.
This study demonstrates that testicular tissue and cells from sexual reassignment patients can be successfully cryopreserved with a clinical-grade procedure and important cell populations are not only preserved but also enriched by the process.
Further studies will determine whether these findings from hormone-treated patients can be generalized to other patients.
American Psychological Association (APA)
Pacchiarotti, Jason& Ramos, Thomas& Howerton, Kyle& Greilach, Scott& Zaragoza, Karina& Olmstead, Marnie…[et al.]. 2013. Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005454
Modern Language Association (MLA)
Pacchiarotti, Jason…[et al.]. Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005454
American Medical Association (AMA)
Pacchiarotti, Jason& Ramos, Thomas& Howerton, Kyle& Greilach, Scott& Zaragoza, Karina& Olmstead, Marnie…[et al.]. Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005454