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A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets
Joint Authors
Spata, Tyler
Bobek, Daniel
Whitson, Bryan A.
Parthasarathy, Sampath
Mohler, Peter J.
Higgins, Robert S. D.
Kilic, Ahmet
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
There is a paucity of a biological large animal model of myocardial infarction (MI).
We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI.
Methods.
Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery.
Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three.
Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic “border zone,” and remote) zones.
Results.
Serial serum troponin levels (μg/mL) measured 0.0±0.0 at baseline and peaked at 297.4±58.0 on post-embolization day 1, followed by 153.0±38.8 on day 2 and 76.7±19.8 on day 3.
Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis.
Conclusion.
We report a reproducible, unique model of a biological myocardial infarction in a large animal model.
This technique can be used to study acute, regional myocardial changes following a thrombotic injury.
American Psychological Association (APA)
Spata, Tyler& Bobek, Daniel& Whitson, Bryan A.& Parthasarathy, Sampath& Mohler, Peter J.& Higgins, Robert S. D.…[et al.]. 2013. A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005474
Modern Language Association (MLA)
Spata, Tyler…[et al.]. A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1005474
American Medical Association (AMA)
Spata, Tyler& Bobek, Daniel& Whitson, Bryan A.& Parthasarathy, Sampath& Mohler, Peter J.& Higgins, Robert S. D.…[et al.]. A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005474