Rotational Transport of Islets: The Best Way for Islets to Get around?
Joint Authors
Oberhuber, Rupert
Mittermair, Christof
Zelger, Bettina
Pirkebner, Daniela
Draxl, Anna
Weissenbacher, Annemarie
Resch, Thomas
Margreiter, Christian
Sucher, Robert
Margreiter, Raimund
Pratschke, Johann
Hengster, Paul
Hermann, Martin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Islet transplantation is a valid treatment option for patients suffering from type 1 diabetes mellitus.
To assure optimal islet cell quality, specialized islet isolation facilities have been developed.
Utilization of such facilities necessitates transportation of islet cells to distant institutions for transplantation.
Despite its importance, a clinically feasible solution for the transport of islets has still not been established.
We here compare the functionality of isolated islets from C57BL/6 mice directly after the isolation procedure as well as after two simulated transport conditions, static versus rotation.
Islet cell quality was assessed using real-time live confocal microscopy.
In vivo islet function after syngeneic transplantation was determined by weight and blood sugar measurements as well as by intraperitoneal glucose tolerance tests.
Vascularization of islets was documented by fluorescence microscopy and immunohistochemistry.
All viability parameters documented comparable cell viability in the rotary group and the group transplanted immediately after isolation.
Functional parameters assessed in vivo displayed no significant difference between these two groups.
Moreover, vascularization of islets was similar in both groups.
In conclusion, rotary culture conditions allows the maintenance of highest islet quality for at least 15 h, which is comparable to that of freshly isolated islets.
American Psychological Association (APA)
Oberhuber, Rupert& Mittermair, Christof& Zelger, Bettina& Pirkebner, Daniela& Draxl, Anna& Weissenbacher, Annemarie…[et al.]. 2013. Rotational Transport of Islets: The Best Way for Islets to Get around?. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005555
Modern Language Association (MLA)
Oberhuber, Rupert…[et al.]. Rotational Transport of Islets: The Best Way for Islets to Get around?. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1005555
American Medical Association (AMA)
Oberhuber, Rupert& Mittermair, Christof& Zelger, Bettina& Pirkebner, Daniela& Draxl, Anna& Weissenbacher, Annemarie…[et al.]. Rotational Transport of Islets: The Best Way for Islets to Get around?. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1005555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005555