Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
Joint Authors
Doucet, Julie
Zhao, An
Fu, Jean
Avrameas, Alexandre
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established.
The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins.
The assay was validated and was shown to be accurate and precise over the entire quantification range (0.59 to 68.4 pg/mL in human EDTA plasma).
The validated assay was successfully applied to samples from healthy volunteers and patients with atopic seasonal rhinitis.
The assay is suitable for use in clinical trials to monitor efficacy or pharmacodynamic effects of drug candidates.
American Psychological Association (APA)
Doucet, Julie& Zhao, An& Fu, Jean& Avrameas, Alexandre. 2013. Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum. Disease Markers،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005810
Modern Language Association (MLA)
Doucet, Julie…[et al.]. Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum. Disease Markers No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1005810
American Medical Association (AMA)
Doucet, Julie& Zhao, An& Fu, Jean& Avrameas, Alexandre. Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1005810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005810