Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism
Joint Authors
Zhu, Qiang
Shen, Jian
Li, Guoqiang
Zhang, Feng
Lu, Ling
Liu, Xiaoshan
Qian, Xiaofeng
Shi, Weidong
Gu, Jian
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To investigate the relationship between interleukin-17 and proteins involved in fatty acid metabolism with respect to alcoholic liver disease, male ICR mice were randomized into five groups: control, alcoholic liver disease (ALD) at 4 weeks, 8 weeks, and 12 weeks, and anti-IL-17 antibody treated ALD.
A proteomic approach was adopted to investigate changes in liver proteins between control and ALD groups.
The proteomic analysis was performed by two-dimensional difference gel electrophoresis.
Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification.
Additionally, expression levels of selected proteins were confirmed by western blot.
Transcriptional levels of some selected proteins were determined by RT-PCR.
Expression levels of 95 protein spots changed significantly (ratio >1.5, P<0.05) during the development of ALD.
Sterol regulatory element-binding protein-lc (SREBP-1c), carbohydrate response element binding protein (ChREBP), enoyl-coenzyme A hydratase (ECHS1), and peroxisome proliferator-activated receptor alpha (PPAR-α) were identified by MS/MS among the proteins shown to vary the most; increased IL-17 elevated the transcription of SREBP-1c and ChREBP but suppressed ECHS1 and PPAR-α.
The interleukin-17 signaling pathway is involved in ALD development; anti-IL-17 antibody improved hepatic steatosis by suppressing interleukin-17-related fatty acid metabolism.
American Psychological Association (APA)
Shi, Weidong& Zhu, Qiang& Gu, Jian& Liu, Xiaoshan& Lu, Ling& Qian, Xiaofeng…[et al.]. 2013. Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1006504
Modern Language Association (MLA)
Shi, Weidong…[et al.]. Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism. Journal of Immunology Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1006504
American Medical Association (AMA)
Shi, Weidong& Zhu, Qiang& Gu, Jian& Liu, Xiaoshan& Lu, Ling& Qian, Xiaofeng…[et al.]. Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1006504
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1006504