The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Joint Authors
Andréasson, Emil
Önnheim, Karin
Forsman, Huamei
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The localization in neutrophils, of the receptor for platelet-activating factor (PAFR), has been determined using subcellular fractionation and a receptor mobilization protocol.
We show that the PAFR is expressed primarily in the plasma membrane.
Although activation of neutrophils by PAF induces responses typical also of agonists that bind the formyl peptide receptors (FPR), known to be stored in mobilizable organelles, some quantitative as well as qualitative differences were observed when neutrophils were activated through these receptors.
PAF is equipotent to fMLF (high affinity agonist for FPR1) to cleave off L-selectin and to induce granule/vesicle secretion but is more potent than fMLF to induce a rise in intracellular Ca2+.
Similar to fMLF, PAF induced also a robust release of reactive oxygen species, but with higher EC50 value and was less sensitive to a PI3K inhibitor compared to the fMLF response.
Despite the lack of a granule localized storage pool of receptors, the PAF-induced superoxide production could be primed; receptor mobilization was, thus, not required for priming of the PAF response.
The desensitized PAFR could not be reactivated, suggesting that distinct signaling pathways are utilized for termination of the responses triggered through FPR1 and PAFR.
American Psychological Association (APA)
Andréasson, Emil& Önnheim, Karin& Forsman, Huamei. 2013. The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1006745
Modern Language Association (MLA)
Andréasson, Emil…[et al.]. The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics. Journal of Immunology Research No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1006745
American Medical Association (AMA)
Andréasson, Emil& Önnheim, Karin& Forsman, Huamei. The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1006745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1006745