A HumanMurine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro
Joint Authors
Duesbery, Nicholas S.
Chen, Ximin
Cao, Brian
Cheng, Xunjia
Zhu, Jin
Ding, Guipeng
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human anthrax infection caused by exposure to Bacillus anthracis cannot always be treated by antibiotics.
This is mostly because of the effect of the remaining anthrax toxin in the body.
Lethal factor (LF) is a component of lethal toxin (LeTx), which is the major virulence of anthrax toxin.
A murine IgG monoclonal antibody (mAb) against LF with blocking activity (coded LF8) was produced in a previous study.
In this report, a human/murine chimeric Fab mAb (coded LF8-Fab) was developed from LF8 by inserting murine variable regions into human constant regions using antibody engineering to reduce the incompatibility of the murine antibody for human use.
The LF8-Fab expressed in Escherichia coli could specifically identify LF with an affinity of 3.46×107 L/mol and could neutralize LeTx with an EC50 of 85 μg/mL.
Even after LeTx challenge at various time points, the LF8-Fab demonstrated protection of J774A.1 cells in vitro.
The results suggest that the LF8-Fab might be further characterized and potentially be used for clinical applications against anthrax infection.
American Psychological Association (APA)
Ding, Guipeng& Chen, Ximin& Zhu, Jin& Duesbery, Nicholas S.& Cheng, Xunjia& Cao, Brian. 2013. A HumanMurine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1006761
Modern Language Association (MLA)
Ding, Guipeng…[et al.]. A HumanMurine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro. Journal of Immunology Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1006761
American Medical Association (AMA)
Ding, Guipeng& Chen, Ximin& Zhu, Jin& Duesbery, Nicholas S.& Cheng, Xunjia& Cao, Brian. A HumanMurine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1006761
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1006761