Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis
Joint Authors
Radzimski, Christiane
Schlumberger, Wolfgang
Blöcker, Inga Madeleine
Bogdanos, Dimitrios P.
Teegen, Bianca
Komorowski, Lars
Rentzsch, Kristin
Probst, Christian
Dähnrich, Cornelia
Stöcker, Winfried
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Autoantibodies against soluble liver antigen (SLA) are specific markers for autoimmune hepatitis (AIH) type 1.
In contrast to the determination of other AIH-associated autoantibodies by indirect immunofluorescence assay (IFA), detection of anti-SLA relied up to now on ELISA or immunoblot based on bacterially expressed recombinant protein.
In order to develop a complementary IFA substrate, SLA isoform 1 was recombinantly produced in the human cell line HEK293 and controlled by a rabbit hyperimmune serum against SLA.
The recombinant cells were used in IFA (RC-IFA) to analyze sera from 20 AIH patients with anti-SLA positivity predetermined by ELISA together with 80 controls (20 anti-SLA negative AIH, 15 primary biliary cirrhosis, 15 HCV, and 30 healthy blood donors).
Using RC-IFA, anti-SLA was detected in all ELISA positive AIH sera but in none of the controls.
Furthermore, a cytosolic fraction of HEK293 containing SLA was able to neutralize the autoantibodies in all positive sera in a dose-dependent manner.
HEK293 cells expressing SLA are a valid substrate for the serodiagnosis of AIH relevant autoantibodies by IFA.
In concert with cryosections of primate liver, rat kidney, rat liver, rat stomach, and HEp-2 cells, they enable the parallel determination of all autoantibodies associated with autoimmune liver diseases.
American Psychological Association (APA)
Radzimski, Christiane& Probst, Christian& Teegen, Bianca& Rentzsch, Kristin& Blöcker, Inga Madeleine& Dähnrich, Cornelia…[et al.]. 2013. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1006803
Modern Language Association (MLA)
Radzimski, Christiane…[et al.]. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Journal of Immunology Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1006803
American Medical Association (AMA)
Radzimski, Christiane& Probst, Christian& Teegen, Bianca& Rentzsch, Kristin& Blöcker, Inga Madeleine& Dähnrich, Cornelia…[et al.]. Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1006803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1006803