Rapamycin Ameliorates Proteinuria and Restores Nephrin and Podocin Expression in Experimental Membranous Nephropathy
Joint Authors
Stylianou, Konstantinos
Nakopoulou, Lydia
Stratigis, Spyros
Daphnis, Eugene
Vardaki, Eleftheria
Moisiadis, Demitrios
Petra, Christina
Stratakis, Stavros
Petrakis, Ioannis
Mavroeidi, Vasiliki
Poulidaki, Rafaela
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Recent studies have shown a beneficial effect of rapamycin in passive and active Heymann Nephritis (HN).
However, the mechanisms underlying this beneficial effect have not been elucidated.
Methods.
Passive Heymann Nephritis (PHN) was induced by a single intravenous infusion of anti-Fx1 in 12 Sprague-Dawley male rats.
One week later, six of these rats were commenced on daily treatment with subcutaneous rapamycin 0.5 mgr/kg (PHN-Rapa).
The remaining six rats were used as the proteinuric control group (PHN) while six more rats without PHN were given the rapamycin solvent and served as the healthy control group (HC).
All rats were sacrificed at the end of the 7th week.
Results.
Rapamycin significantly reduced proteinuria during the autologous phase of PHN.
Histological lesions were markedly improved by rapamycin.
Immunofluorescence revealed attenuated deposits of autologous alloantibodies in treated rats.
Untreated rats showed decreased glomerular content of both nephrin and podocin whereas rapamycin restored their expression.
Conclusions.
Rapamycin monotherapy significantly improves proteinuria and histological lesions in experimental membranous nephropathy.
This beneficial effect may be mediated by inhibition of the alloimmune response during the autologous phase of PHN and by restoration of the normal expression of the podocyte proteins nephrin and podocin.
American Psychological Association (APA)
Stratakis, Stavros& Stylianou, Konstantinos& Petrakis, Ioannis& Mavroeidi, Vasiliki& Poulidaki, Rafaela& Petra, Christina…[et al.]. 2013. Rapamycin Ameliorates Proteinuria and Restores Nephrin and Podocin Expression in Experimental Membranous Nephropathy. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1007014
Modern Language Association (MLA)
Stratakis, Stavros…[et al.]. Rapamycin Ameliorates Proteinuria and Restores Nephrin and Podocin Expression in Experimental Membranous Nephropathy. Journal of Immunology Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1007014
American Medical Association (AMA)
Stratakis, Stavros& Stylianou, Konstantinos& Petrakis, Ioannis& Mavroeidi, Vasiliki& Poulidaki, Rafaela& Petra, Christina…[et al.]. Rapamycin Ameliorates Proteinuria and Restores Nephrin and Podocin Expression in Experimental Membranous Nephropathy. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1007014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1007014