![](/images/graphics-bg.png)
Preparation, Characterization, and Cytotoxicity of Various Chitosan Nanoparticles
Joint Authors
Yao, Qian
Liu, Wei
Gou, Xiao-Jun
Guo, Xiao-Qiang
Yan, Jun
Song, Qin
Chen, Feng-Zheng
Zhao, Qi
Chen, Chao
Chen, Tian
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Chitosan nanoparticles (CS-NPs) without drug loading have diverse biological activity.
In this study, we prepared CS-NPs, CS-NPs solidified by different amount of glutaraldehyde, and CS-NPs modified with either biotin (B-CS-NPs) or biotin and avidin (A-B-CS-NPs) and examined their cytotoxicity on HepG2 cells.
The morphology and size were measured by transmission electron microscopy and photon correction spectroscopy, respectively.
The extent of solidification was validated by the approach of sonication.
Biotin connect density on the surface of B-CS-NPs and A-B-CS-NPs was determined by biotin assay kit.
The results showed that most of the NPs were round and their mean sizes were all below 300 nm.
Biotin connect density of B-CS-NPs and A-B-CS-NPs was 2.18 ± 0.36 and 1.26 ± 0.11 mol biotin/mol CS, respectively.
At relatively low concentration, CS-NPs with higher extent of solidification exhibited more vigorous inhibitory effect against HepG2 cells than those without solidification.
When NPs were incubated with cancer cells for 48 h, compared with CS-NPs, the anticancer activity of B-CS-NPs and A-B-CS-NPs was enhanced significantly (P<0.05).
In addition, A-B-CS-NPs showed superior cytotoxicity over B-CS-NPs.
This study demonstrates that modification with biotin and avidin may be an efficient way in improving antitumor activity of CS-NPs against hepatic carcinoma.
American Psychological Association (APA)
Yao, Qian& Liu, Wei& Gou, Xiao-Jun& Guo, Xiao-Qiang& Yan, Jun& Song, Qin…[et al.]. 2013. Preparation, Characterization, and Cytotoxicity of Various Chitosan Nanoparticles. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1007343
Modern Language Association (MLA)
Yao, Qian…[et al.]. Preparation, Characterization, and Cytotoxicity of Various Chitosan Nanoparticles. Journal of Nanomaterials No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1007343
American Medical Association (AMA)
Yao, Qian& Liu, Wei& Gou, Xiao-Jun& Guo, Xiao-Qiang& Yan, Jun& Song, Qin…[et al.]. Preparation, Characterization, and Cytotoxicity of Various Chitosan Nanoparticles. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1007343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1007343