Multiplex Immunoassays of Plant Viruses Based on Functionalized Upconversion Nanoparticles Coupled with Immunomagnetic Separation
Joint Authors
Zhang, Mingzhe
Chen, Wujian
Chen, Xi
Zhang, Yongjiang
Lin, Xiaojia
Wu, Zhiyi
Li, Mingfu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A sensitive, specific and rapid method for the detection of three different kinds of plant viruses including tomato ringspot virus (ToRSV), bean pod mottle virus (BPMV) and arabis mosaic virus (ArMV) was demonstrated using novel upconversion nanoparticles (UCNPs) as a fluorescence marker coupled with immunomagnetic separation.
Magnetic nanoparticles (MNPs, ~100 nm) were coated with different antibodies were employed to capture and enrich the target viruses.
Then antibody-conjugated UCNPs as signal probes were added to form sandwich complexes.
This was followed by a fluorescence measurement using a 980 nm laser.
This method not only avoids the difficulty in simultaneous detection of multiple independent organic fluorphores that require distinct excitation wavelengths, and auto-fluorescence of biological samples due to the higher-energy excitation of QDs but also amplifies detection signal by UCNPs-tags together with easy separation of samples by magnetic forces, demonstrating the potential to be used for detecting virus in the field of environmental safety and other fields.
American Psychological Association (APA)
Zhang, Mingzhe& Chen, Wujian& Chen, Xi& Zhang, Yongjiang& Lin, Xiaojia& Wu, Zhiyi…[et al.]. 2013. Multiplex Immunoassays of Plant Viruses Based on Functionalized Upconversion Nanoparticles Coupled with Immunomagnetic Separation. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1007663
Modern Language Association (MLA)
Zhang, Mingzhe…[et al.]. Multiplex Immunoassays of Plant Viruses Based on Functionalized Upconversion Nanoparticles Coupled with Immunomagnetic Separation. Journal of Nanomaterials No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1007663
American Medical Association (AMA)
Zhang, Mingzhe& Chen, Wujian& Chen, Xi& Zhang, Yongjiang& Lin, Xiaojia& Wu, Zhiyi…[et al.]. Multiplex Immunoassays of Plant Viruses Based on Functionalized Upconversion Nanoparticles Coupled with Immunomagnetic Separation. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1007663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1007663