DNA Detection of Toxoplasma gondii with a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor
Joint Authors
Xu, Shichao
Zhang, Chen
He, Lei
Wang, Tongyao
Ni, Liusong
Sun, Mengna
Miao, Heng
Zhang, Jimei
Dai, Zhao
Wang, Bing
Zheng, Guo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A new method for detection of Toxoplasma gondii via DNA sensing technology was developed in this study.
It was based on the mechanism of fluorescence resonance energy transfer (FRET) in which multifunctional and magnetic-fluorescent CdTe@Ni quantum dots (mQDs) were utilized as energy donor and a commercial BHQ2 as acceptor.
The sensing probe was fabricated by labeling a stem-loop Toxoplasma gondii DNA oligonucleotide with CdTe@Ni mQDs at the 5′ end and BHQ2 at 3′ end, respectively.
The surface assembly of CdTe on Ni core and the formation of CdTe@Ni were confirmed by XRD analysis.
The sizes of CdTe, Ni nanoparticles, and CdTe@Ni were measured via TEM and XRD methods and estimated to be ~3 nm, ~15 nm, and ~20 nm, respectively.
The sensing ability was investigated by the fluorescence spectrum (FS).
An obvious fluorescence recovery was observed when the complete complimentary target Toxoplasma gondii DNA was introduced, which did not happen in the case of the target DNA with one-base pair mismatch.
Our research indicates that the current sensing probe is sensitive and specific in detection of Toxoplasma gondii DNA and has great potential in Toxoplasmosis diagnosis.
American Psychological Association (APA)
Xu, Shichao& Zhang, Chen& He, Lei& Wang, Tongyao& Ni, Liusong& Sun, Mengna…[et al.]. 2013. DNA Detection of Toxoplasma gondii with a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1007818
Modern Language Association (MLA)
Xu, Shichao…[et al.]. DNA Detection of Toxoplasma gondii with a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor. Journal of Nanomaterials No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1007818
American Medical Association (AMA)
Xu, Shichao& Zhang, Chen& He, Lei& Wang, Tongyao& Ni, Liusong& Sun, Mengna…[et al.]. DNA Detection of Toxoplasma gondii with a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1007818
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1007818