Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering
Joint Authors
Liou, Hau-Min
Rau, Lih-Rou
Huang, Chun-Chiang
Lu, Meng-Ru
Hsu, Fu-Yin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Schwann cells play a critical role in the repair of the peripheral nerve.
The goal of this study was to fabricate electrospun gelatin (Gel) and hyaluronan-gelatin (HA-Gel) composite nanofibers to provide a suitable growth environment for Schwann cells.
The fiber diameters of Gel, 0.5 HA-Gel, 1 HA-Gel, and 1.5 HA-Gel were 130 ± 30 nm, 294 ± 87 nm, 362 ± 129 nm, and 224 ± 54 nm, respectively.
The biological performance of Gel and HA-Gel was evaluated using an in vitro culture of RT4-D6P2T rat Schwann cells.
We found that the cell attachment and proliferation rates were not significantly different on these matrices.
However, the Schwann cells displayed better organized F-actin on HA-Gel than on Gel.
Moreover, the expression levels of several genes, including Nrg1, GFAP, and P0, were significantly higher on HA-Gel than on Gel.
In addition, the levels of Nrg1 and P0 protein expression were also higher on the HA-Gel than on Gel.
These results indicate that the hyaluronan-gelatin composite nanofibrous matrix could potentially be used in peripheral nerve repair.
American Psychological Association (APA)
Liou, Hau-Min& Rau, Lih-Rou& Huang, Chun-Chiang& Lu, Meng-Ru& Hsu, Fu-Yin. 2013. Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1007984
Modern Language Association (MLA)
Liou, Hau-Min…[et al.]. Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering. Journal of Nanomaterials No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1007984
American Medical Association (AMA)
Liou, Hau-Min& Rau, Lih-Rou& Huang, Chun-Chiang& Lu, Meng-Ru& Hsu, Fu-Yin. Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1007984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1007984