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Facile and Sensitive Epifluorescent Silica Nanoparticles for the Rapid Screening of EHEC
Joint Authors
Songvorawit, Nut
Tuitemwong, Kooranee
Tuitemwong, Pravate
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study was to develop antibodies conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) aiming to increase signals for the rapid detection of Escherichia coli O157:H7 with glass slide method.
The FDS-NPs were produced with microemulsion/sol-gel techniques resulting in spherical in shape with 47 ± 6 nm in diameter.
The particles showed high intensity and stable orange color Rubpy luminescent dye.
The XRD spectrum showed a broad diffraction peak in the range of 18–30∘ (centered at 22∘) indicating an amorphous structure.
Surface modifications for bioconjugation with affinity chromatography purified (IgGs) antibodies were successful.
The properties were evident from FTIR spectra at 1631.7 cm−1.
Results indicated that nanoparticles could attach onto cells of E.
coli O157:H7 coated on a glass slide, and give distinctively bright color under epifluorescence microscope (400x).
It was shown that FDS-NPs could detect a very low amount of cells of E.
coli O157:H7 (16 CFU in 10 ml) in 60 min.
The phosphate buffered saline (PBS) with ionic strength of 1.70 gave zeta potential of good particle dispersion (−40 mV).
This work demonstrated that highly sensitive bioconjugated E.
coli O157:H7 FDS-NPs were successfully developed with a potential to be used for the rapid detection of E.
coli O157:H7 in foods.
American Psychological Association (APA)
Tuitemwong, Pravate& Songvorawit, Nut& Tuitemwong, Kooranee. 2013. Facile and Sensitive Epifluorescent Silica Nanoparticles for the Rapid Screening of EHEC. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1008094
Modern Language Association (MLA)
Tuitemwong, Pravate…[et al.]. Facile and Sensitive Epifluorescent Silica Nanoparticles for the Rapid Screening of EHEC. Journal of Nanomaterials No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1008094
American Medical Association (AMA)
Tuitemwong, Pravate& Songvorawit, Nut& Tuitemwong, Kooranee. Facile and Sensitive Epifluorescent Silica Nanoparticles for the Rapid Screening of EHEC. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1008094
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1008094