Immunological effects of some antigens and DNA synthetic response on male rabbits

Abstract EN

Seventy-two Californian male rabbits (CL) were randomly selected at 5.5 months old.

They were divided into for equal four groups (18 rabbits each) according to results of immunization which they immunized four immunizations by four antigens : sheep red blood cells ( SRBCs ) ,bovine serum albumin ( BSA ),Rabbit gamma globulin (RgG ), and phospho.

L.tyrosine, (ph.

L.

ty), for group 1, 2, 3 and 4, respectively.

Each group was injected four immunizations with its antigen and with adjuvant : first was antigen with Freund's complete (FC ) , the second was antigen with incomplete ( FI ) ,the third was antigen with fluorescing isothio cyanate ( FITC ) conjugated to human serum albumin ( HAS ) and the last immunization with 5- iodo-2-deoxyuridine ( I125 UDR ) .

Rabbits were injected subcauteneously in first immunization, after 28 days the second immunization was intravenously.

Also, 28 days later all rabbits groups were injected the third immunization in foot pad, after 15 days from the third immunization all rabbits were injected intravenously with the last immunization.

Blood samples were taken from marginal ear vein intervals, 7, 14 , 21- day post -first and second immunization to determine the serological tests (antibody assay and titration ) and rabbits were classified later into high (HR) and low response (LR) responded groups as a result of immune response.

The low responder rabbits were culled of the experiment.

At the end of experiment rabbits were sacrificed and blood samples were taken to estimate physiological and immunological traits.

Also, semen characteristics and DNA synthesis of thymocyte tissues, PCR-DNA amplification with specific primer was carried out to study sequence of IgH thymocyte.

The results of serological traits showed that there were significant differences (p < 0.05) among rabbit groups, group (1) had superiority antibodies responses values and the lowest value was group (4).

Physiological parameters studied (TP, Alb, glo and A / G ratio) showed that there were significant differences ( p < 0.05 ) among rabbit groups, group (3) had higher values and the lowest was group (1).

Results of immunological traits : { (TWBCs and its differentiation, and total immunoglobulins (Ig) and its fractions Alpha immunoglobulin (IgA), Meu immunoglobulin (IgM) and Gamma (IgG)} showed that TWBCs and its differentiation were highly significant differences ( p < 0.01 ) among rabbit groups, group (1) and (3) had superiority values of (TWBCs) and its differentiation counts.

The results of Ig were : group (3) had higher value of total Ig followed by group (4), but group (1) had lower value of Ig, whenever group (2) had intermediate value and there was significant differences (P < 0.05) among rabbit groups.

Concerning of Ig fractions (IgA, IgM and IgG), there were significant differences (P < 0.05) among rabbit groups the greatest value of IgA was detected in serum of group (4), but the lower value was for group (1).

The highest value of IgM was in group (1).

Group (3) had higher value of IgG than other groups.

Concerning of DNA amplification there differences between bands according to the effect of different antigens and its stimulating to the IgH genes.

Regarding semen characteristics there was a high significant differences (p < 0.01) among immunized rabbit groups, group (4) achieved the lowest value of dead and abnormal spermatozoa and the highest value of semen motility than other groups.

It could be concluded that phospho - L.

ty material had ability to stimulate DNA and induce immune response, besides increasing fertility of male rabbits.