![](/images/graphics-bg.png)
Rapamycin Inhibits ALDH Activity, Resistance to Oxidative Stress, and Metastatic Potential in Murine Osteosarcoma Cells
Joint Authors
Schott, Trevor
Weiss, Kurt
Isaac, Christian
Mu, Xiaodong
Huard, Johnny
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Osteosarcoma (OS) is the most common primary malignancy of bone.
Mortality is determined by the presence of metastatic disease, but little is known regarding the biochemical events that drive metastases.
Two murine OS cell lines, K7M2 and K12, are related but differ significantly in their metastatic potentials: K7M2 is highly metastatic whereas K12 displays much less metastatic potential.
Using this experimental system, the mammalian target of rapamycin (mTOR) pathway has been implicated in OS metastasis.
We also discovered that aldehyde dehydrogenase (ALDH, a stem cell marker) activity is higher in K7M2 cells than K12 cells.
Rapamycin treatment reduces the expression and enzymatic activity of ALDH in K7M2 cells.
ALDH inhibition renders these cells more susceptible to apoptotic death when exposed to oxidative stress.
Furthermore, rapamycin treatment reduces bone morphogenetic protein-2 (BMP2) and vascular endothelial growth factor (VEGF) gene expression and inhibits K7M2 proliferation, migration, and invasion in vitro.
Inhibition of ALDH with disulfiram correlated with decreased mTOR expression and activity.
In conclusion, we provide evidence for interaction between mTOR activity, ALDH activity, and metastatic potential in murine OS cells.
Our work suggests that mTOR and ALDH are therapeutic targets for the treatment and prevention of OS metastasis.
American Psychological Association (APA)
Mu, Xiaodong& Isaac, Christian& Schott, Trevor& Huard, Johnny& Weiss, Kurt. 2013. Rapamycin Inhibits ALDH Activity, Resistance to Oxidative Stress, and Metastatic Potential in Murine Osteosarcoma Cells. Complexity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1011452
Modern Language Association (MLA)
Mu, Xiaodong…[et al.]. Rapamycin Inhibits ALDH Activity, Resistance to Oxidative Stress, and Metastatic Potential in Murine Osteosarcoma Cells. Complexity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1011452
American Medical Association (AMA)
Mu, Xiaodong& Isaac, Christian& Schott, Trevor& Huard, Johnny& Weiss, Kurt. Rapamycin Inhibits ALDH Activity, Resistance to Oxidative Stress, and Metastatic Potential in Murine Osteosarcoma Cells. Complexity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1011452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1011452